The EcoGenomics and BioInformatics Lab (EBL) and BioMolecular Resource Facility (BRF) are planning to run a facilitated RNA Sequencing and associated BioInformatics course at ANU around March next year, and are calling for an expression of interest (EOI) in participating.
The exact design of the workshop will depend on the EOI responses but will likely involve:
- A wet lab component with participants bringing their own high-quality RNA “ready to go”.
A dry lab / computational component providing the tools for data analysis; specifically
- Transcriptome assembly for non-model species
- Differential gene expression analysis
The course will be best suited to participants who (will) have a project underway with RNA ready to sequence. To ensure that participants get the most out of the course, we will endeavour to tailor the course as much as possible around participants’ requirements. To that end, we have a few questions…
Specifically, we’re interested in your timeframe, i.e. when would you have your field / lab experiment done and RNA ready to go? The name of your species and experimental design; i.e. brief description of the experiment, the question(s) being asked, number of conditions and samples. (The intended methodology will be polyA /mRNA based so be suited to eukaryotes only).
Further, we would also need to know if there is a reference genome or transcriptome available for your species or you will need to make the reference directly from the RNASeq data (i.e. perform a de-novo transcriptome assembly).
We expect the course to take place over 3-4 weeks (depending on the answers to the above), though there would likely be significant “down time” for participants to work on unrelated tasks. For example, Illumina sequencing will take up to a week and several of the data analysis steps may take 24-48 hours of computation.
There will be a cost associated with the course to cover RNA-seq reagents and sequencing. This will likely be heavily discounted but may still be in the ballpark of $2-3000 depending on methods and sequencing platform (i.e. your EOI responses).
Participants would require reasonable level of lab and computer skills, i.e. it will not really be suitable for inexperienced novices in genomic analysis.
If you think you would be interested in either or both of the wet or dry components, it would be great if you could complete the Expression of Interest form by 21 December, 2018.