This CBA-supported workshop is targeted at beginner and intermediate users of the Oxford Nanopore MinION sequencing platform. It represents a unique opportunity to learn more about this exciting and mature sequencing platform from experienced users.
The hands-on workshop will be run over three days, covering the technology, DNA sample QC, library preparation techniques (rapid, 1D and 1D^2), starting runs, bioinformatics analysis and interpretation.
Applications to attend are now open and will close 1 May 2017. Ten places are reserved for local researchers at ANU while another 20 are open to the wider sequencing community at CSIRO, University Canberra and elsewhere. All applications are strictly competitive and aimed at ensuring diversity.
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- Oxford Nanopore - How nanopore sequencing works
- Poretools: a toolkit for working with nanopore sequencing data
- NanoOK - Multi-reference alignment analysis
- Learn the basics of DNA extraction and DNA QC obtained from (non-)model organisms.
- Develop best wet-lab practices to produce a high-quality MinION library.
- Hands-on experience, running your MinION on your own computer.
- Discover suitable data-handling methods for MinION output.
- Discuss latest bioinformatic methods for analysis of real-time nanopore data.
- Day 1: Background, DNA sample QC, library prep, starting your run.
- Day 2: Bioinformatic background and initial analysis of your run.
- Day 3: Bringing it all together and finishing up.
How to apply
- Fill in the expression-of-interest form. Successful participants will be notified by 5 May.
- Attendance fee: $900 AUD (includes flowcell & library kit for MinION run and catering).
- You must BYO MinION device and a compatible laptop.
Workshop organisers (ANU)
- Benjamin Schwessinger
- Megan McDonald
- Steve Eichten
- Norman Warthmann
- Benjamin Schwessinger (ANU)
- Megan McDonald (ANU)
- Steve Eichten (ANU)
- Louise Judd (UniMelb)
- ANU Research School of Biology
- ANU EcoGenomics Lab
- ANU Centre for Biodiversity Analysis
- The Centre for Genomics, Metabolomics and Bioinformatics
- ARC Centre of Excellence in Plant Energy Biology
- Oxford Nanopore